Primary Details
| Project ID | PTP005 |
| Transcriptome Exp. ID | PTE020 |
| Plant Name | Arabidopsis thaliana |
| Genome ID |
Plant Classification
|
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| Kingdom | Plantae | |
| Phylum | Streptophyta | |
| Class | Magnolipsida | |
| Order | Brassicales | |
| Family | Brassicaceae | |
| Genus | Arabidopsis | |
| Species | thaliana | |
| Variety | Col. 0 Foldback (miR858) |
Transcriptome Details
Sample Tissue
Leaf
Line/Chemotype/Cultivar
Col. 0 Foldback (miR858)
Study Title
Functional characterization of miR858 from Arabidopsis thaliana
Description
miR858 is a potential regulator of the phenylpropanoid pathway and plant development. miR858a targets R2R3-MYB transcription factors involved in flavonoid biosynthesis. miR858OX plants show robust growth and early flowering in contrast to the reduction of plant growth and delayed flowering observed in Arabidopsis transgenic lines expressing an artificial miRNA target mimic (MIM858). In this project, the global changes occurring in the rosette and stem tissues of miR858OX and MIM858 were analyzed through transcriptome analysis. The study identified a large number of differentially expressed genes related to growth and development in miR858OX and MIM858 lines compared to WT plants. A number of differentially expressed genes encoding small peptides were also identified in the study.
Sequencing Platform
Illumina HiSeq 2500
Sequencing Strategy
RNA-Seq
Sequencing Library
Single
Experiment Type
Transgenic
Replicate Number
1
Total Replicates
1
Merged replicated
0
Library Name
FR2
Selection Method
PCR
Number of run
1
Spots No
19978891
Bases no
4.0
Spot Length
200
Submitted By
Dr. Prabodh Kumar Trivedi
Affliated Institute
CSIR-Central Institute Of Medicinal And Aromatic Plants
Country
India
State
Uttar Pradesh
City
Lucknow
SRA (NCBI) Project ID
SRA (NCBI) Experiment ID
SRA (NCBI) Sample ID
SRA (NCBI) Run ID
Other Related Entries
| Project ID | Experiment ID | Plant Name | Sample/Tissue | Exp. Type | Replicate No. | Total Replicates No. | Merged Assembly (Replicates) | DEGs (Pair) |
|---|---|---|---|---|---|---|---|---|
| PTP005 | PTE016 | Arabidopsis thaliana | Stem | Transgenic | 1 | 1 | 1 | - |
| PTP005 | PTE017 | Arabidopsis thaliana | Stem | Transgenic | 1 | 1 | 1 | - |
| PTP005 | PTE018 | Arabidopsis thaliana | Stem | Control | 1 | 1 | 0 | - |
| PTP005 | PTE019 | Arabidopsis thaliana | Leaf | Transgenic | 1 | 1 | 0 | - |
| PTP005 | PTE020 | Arabidopsis thaliana | Leaf | Transgenic | 1 | 1 | 0 | - |
| PTP005 | PTE021 | Arabidopsis thaliana | Leaf | Control | 1 | 1 | 0 | - |
Reference/Publication Details
| Sr. No | Publication details |
|---|---|
| 1 | Sharma D, Tiwari M, Pandey A, Bhatia C, Sharma A, Trivedi PK. MicroRNA858 Is a Potential Regulator of Phenylpropanoid Pathway and Plant Development. Plant Physiol. 2016 Jun;171(2):944-59. doi: 10.1104/pp.15.01831. Epub 2016 Apr 27. PMID: 27208307; PMCID: PMC4902582. |