Primary Details
| Project ID | PTP005 |
| Transcriptome Exp. ID | PTE016 |
| Plant Name | Arabidopsis thaliana |
| Genome ID |
Plant Classification
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| Kingdom | Plantae | |
| Phylum | Streptophyta | |
| Class | Magnolipsida | |
| Order | Brassicales | |
| Family | Brassicaceae | |
| Genus | Arabidopsis | |
| Species | thaliana | |
| Variety |
Transcriptome Details
Sample Tissue
Stem
Line/Chemotype/Cultivar
Col. 0 Sponge (MIMIC858)
Study Title
Functional characterization of miR858 from Arabidopsis thaliana
Description
miR858 is a potential regulator of the phenylpropanoid pathway and plant development. miR858a targets R2R3-MYB transcription factors involved in flavonoid biosynthesis. miR858OX plants show robust growth and early flowering in contrast to the reduction of plant growth and delayed flowering observed in Arabidopsis transgenic lines expressing an artificial miRNA target mimic (MIM858). In this project, the global changes occurring in the rosette and stem tissues of miR858OX and MIM858 were analyzed through transcriptome analysis. The study identified a large number of differentially expressed genes related to growth and development in miR858OX and MIM858 lines compared to WT plants. A number of differentially expressed genes encoding small peptides were also identified in the study.
Sequencing Platform
Illumina HiSeq 2500
Sequencing Strategy
RNA-Seq
Sequencing Library
Single
Experiment Type
Replicate Number
Total Replicates
Merged replicated
Library Name
SS6
Selection Method
PCR
Number of run
Spots No
20022819
Bases no
4G
Spot Length
200
Submitted By
Dr. Prabodh Kumar Trivedi
Affliated Institute
CSIR-Central Institute of Medicinal and Aromatic Plants
Country
India
State
Uttar Pradesh
City
Lucknow
Raw Reads Downlaod (.fastq)
Download Raw RNA-Seq Data (.fastq)
Raw Data QC Report (FastQC)
Gene Ontology (GO) mapping results details
De-Novo Transcriptome Assembly Data
Plants Enzymes/CYP-P450s/GTs/BGLU Details
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Enrichment Analysis (KEGG/GO)
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Post-Assembly Quality Check Report
InterproScan Results for Protein Families/Motifs/Domains
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De novo assembly N50 statistics report
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InterproScan Results for Protein Families/Motifs/Domains
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Pathway genes
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