Transcriptome



Primary Details

Project ID PTP005
Transcriptome Exp. ID PTE017
Plant Name Arabidopsis thaliana
Genome ID

Plant Classification

Kingdom Plantae
Phylum Streptophyta
Class Magnolipsida
Order Brassicales
Family Brassicaceae
Genus Arabidopsis
Species thaliana
Variety

Transcriptome Details

Sample Tissue

Stem

Line/Chemotype/Cultivar

Col. 0 Foldback (miR858)

Study Title

Functional characterization of miR858 from Arabidopsis thaliana

Description

miR858 is a potential regulator of the phenylpropanoid pathway and plant development. miR858a targets R2R3-MYB transcription factors involved in flavonoid biosynthesis. miR858OX plants show robust growth and early flowering in contrast to the reduction of plant growth and delayed flowering observed in Arabidopsis transgenic lines expressing an artificial miRNA target mimic (MIM858). In this project, the global changes occurring in the rosette and stem tissues of miR858OX and MIM858 were analyzed through transcriptome analysis. The study identified a large number of differentially expressed genes related to growth and development in miR858OX and MIM858 lines compared to WT plants. A number of differentially expressed genes encoding small peptides were also identified in the study.

Sequencing Platform

Illumina HiSeq 2500

Sequencing Strategy

RNA-Seq

Sequencing Library

Single

Experiment Type

Replicate Number

Total Replicates

Merged replicated

Library Name

FS5

Selection Method

PCR

Number of run

Spots No

16723779

Bases no

2G

Spot Length

120

Submitted By

Dr. Prabodh Kumar Trivedi

Affliated Institute

CSIR-Central Institute of Medicinal and Aromatic Plants

Country

India

State

Uttar Pradesh

City

Lucknow

Raw Reads Downlaod (.fastq)

Download Raw RNA-Seq Data (.fastq)
RNA-Seq Reads File 1 (Forward) Open Link
RNA-Seq Reads File 2 (Reverse) Open Link

Raw Data QC Report (FastQC)

Gene Ontology (GO) mapping results details
CYP-P450s/GTs/BGLU Details Download
KEGG/Reactome/Gramene pathways results Download

De-Novo Transcriptome Assembly Data

Plants Enzymes/CYP-P450s/GTs/BGLU Details
Enrichment Analysis (KEGG/GO) Download

Post-Assembly Quality Check Report

InterproScan Results for Protein Families/Motifs/Domains
De novo assembly N50 statistics report
Overall reads-realignment statistics (Bowtie2) View View
InterproScan Results for Protein Families/Motifs/Domains View View

Pathway genes

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Other Related Entries

Project ID Experiment ID Plant Name Sample/Tissue Exp. Type Replicate No. Total Replicates No. Merged Assembly (Replicates) DEGs (Pair)
PTP005 PTE016 Arabidopsis thaliana Stem -
PTP005 PTE017 Arabidopsis thaliana Stem -