Transcriptome



Primary Details

Project ID PTP035
Transcriptome Exp. ID PTE0118
Plant Name Withania somnifera
Genome ID GEN2

Plant Classification

Kingdom Plantae
Phylum Streptophyta
Class Magnolipsida
Order Solanales
Family Solanaceae
Genus Withania
Species somnifera
Variety Wild, perennial Ecotype

Transcriptome Details

Sample Tissue

Leaf

Line/Chemotype/Cultivar

Wild, perennial Ecotype

Study Title

RNA seq analysis of CWS11, an annual, Nagouri ecotype also known as Ashwagandha of commerce (Withania somnifera)

Description

To assist metabolite targeted breeding and to understand genotype to phenotype relationship for leaf architectural traits and leaf specific withanolides i.e Withaferrin-A, Withanone, Withanolide D and Withanosides leaf tissues have been subjected to RNA seq of strand specific libraries. Illumina HiSeq 2000 NEXTflex Directional RNA-Seq (dUTP based)derived strand specific libraries of cultivated annual inbred CWS11 resulted in 4546.6MB data assembled in 67622 contigs with an average N50 of 2011

Sequencing Platform

NextSeq 500

Sequencing Strategy

RNA-Seq

Sequencing Library

Paired

Experiment Type

Replicate Number

Total Replicates

Merged replicated

Library Name

CWS7_T

Selection Method

PCR

Number of run

Spots No

48,418,755

Bases no

9.8G

Spot Length

202

Submitted By

NA

Other Related Entries

Project ID Experiment ID Plant Name Sample/Tissue Exp. Type Replicate No. Total Replicates No. Merged Assembly (Replicates) DEGs (Pair)
PTP035 PTE0117 Withania somnifera Leaf -
PTP035 PTE0118 Withania somnifera Leaf -