Transcriptome



Primary Details

Project ID PTP035
Transcriptome Exp. ID PTE0117
Plant Name Withania somnifera
Genome ID

Plant Classification

Kingdom Plantae
Phylum Streptophyta
Class Magnolipsida
Order Solanales
Family Solanaceae
Genus Withania
Species somnifera
Variety Nagouri ecotype

Transcriptome Details

Sample Tissue

Leaf

Line/Chemotype/Cultivar

Nagouri ecotype

Study Title

RNA seq analysis of CWS11, an annual, Nagouri ecotype also known as Ashwagandha of commerce (Withania somnifera)

Description

To assist metabolite targeted breeding and to understand genotype to phenotype relationship for leaf architectural traits and leaf specific withanolides i.e Withaferrin-A, Withanone, Withanolide D and Withanosides leaf tissues have been subjected to RNA seq of strand specific libraries. Illumina HiSeq 2000 NEXTflex Directional RNA-Seq (dUTP based)derived strand specific libraries of cultivated annual inbred CWS11 resulted in 4546.6MB data assembled in 67622 contigs with an average N50 of 2011.

Sequencing Platform

Illumina NextSeq 500

Sequencing Strategy

RNA-Seq

Sequencing Library

Paired

Experiment Type

Wild

Replicate Number

1

Total Replicates

1

Merged replicated

1

Library Name

CWS11_T

Selection Method

PCR

Number of run

1

Spots No

50355405

Bases no

10.2

Spot Length

202

Submitted By

Dr.Tripta Jhang

Affliated Institute

CSIR-Central Institute Of Medicinal And Aromatic Plants

Country

India

State

Uttar Pradesh

City

Lucknow

Other Related Entries

Project ID Experiment ID Plant Name Sample/Tissue Exp. Type Replicate No. Total Replicates No. Merged Assembly (Replicates) DEGs (Pair)
PTP035 PTE0117 Withania somnifera Leaf Wild 1 1 1 -