Materials & Method
Whole genome sequencing (WGS) library was prepared with Illumina-compatible NEXTflex Rapid DNA sequencing kit (BIOO Scientific, Austin, Texas, U.S.A.) at Genotypic Technology Pvt. Ltd., Bangalore, India. Briefly, DNA was shearedusing Covaris S2 sonicator (Covaris, Woburn, Massachusetts, USA) to generate approx. fragment size distribution from 300 bp to 500bp.The fragment size distribution was checkedon Agilent TapeStation and subsequently purified using Highprepmagnetic beads (Magbio). Purified fragments were end-repaired, adenylated and ligated to Illumina multiplexbarcode adaptorsas per NEXTFlex Rapid DNAsequencing kit protocol. Adapter-ligatedDNA was purified usingHighprepbeads. Resultant fragments were amplified for 5cyclesof PCR using Illumina-compatible primers provided in the NEXTFlexRapid DNA sequencing kit.Final PCR product (sequencing library) was purified withHighprepbeads, followed by library quality control check. Illumina-compatible sequencing library wasquantified by Qubit fluorometer (Thermo Fisher Scientific, MA, USA) and its fragment size distribution was analyzed on Agilent 2200 Tapestation.