Transcriptome



Primary Details

Project ID PTP006
Transcriptome Exp. ID PTE022
Plant Name Asparagus racemosus
Genome ID

Plant Classification

Kingdom Plantae
Phylum Streptophyta
Class Magnolipsida
Order Asparagales
Family Asparagaceae
Genus Asparagus
Species racemosus
Variety CIM-Shakti

Transcriptome Details

Sample Tissue

Leaf and root

Line/Chemotype/Cultivar

CIM-Shakti

Study Title

Analysis of leaf and root transcriptome of Asparagus racemosus (CIM-Shakti)

Description

The study is focusses on the comparative de-novo transcriptome analysis of root versus leaf tissue and identifying some root specific transcripts involved in saponin biosynthesis using high-throughput next generation transcriptome sequencing.

Sequencing Platform

Illumina HiSeq 2500

Sequencing Strategy

WGS

Sequencing Library

Paired

Experiment Type

Control

Replicate Number

1

Total Replicates

1

Merged replicated

1

Library Name

TruSeq RNA library

Selection Method

PolyA

Number of run

1

Spots No

58953846

Bases no

11.9

Spot Length

202

Submitted By

Dr. Rakesh Kumar Shukla

Affliated Institute

CSIR-Central Institute Of Medicinal And Aromatic Plants

Country

India

State

Uttar Pradesh

City

Lucknow

SRA (NCBI) Project ID

PRJNA235257

SRA (NCBI) Experiment ID

SRX460513

SRA (NCBI) Sample ID

SAMN02585729

SRA (NCBI) Run ID

SRR1156272

Other Related Entries

Project ID Experiment ID Plant Name Sample/Tissue Exp. Type Replicate No. Total Replicates No. Merged Assembly (Replicates) DEGs (Pair)
PTP006 PTE022 Asparagus racemosus Leaf and root Control 1 1 1 -

Reference/Publication Details

Sr. No Publication details
1

Leinonen, R., Sugawara, H., Shumway, M., & International Nucleotide Sequence Database Collaboration. (2010). The sequence read archive. Nucleic acids research39(suppl_1), D19-D21.

2

Upadhyay, S., Phukan, U.J., Mishra, S. et al. De novo leaf and root transcriptome analysis identified novel genes involved in Steroidal sapogenin biosynthesis in Asparagus racemosus. BMC Genomics 15, 746 (2014).

3

Upadhyay, S., Phukan, U.J., Mishra, S. et al. De novo leaf and root transcriptome analysis identified novel genes involved in Steroidal sapogenin biosynthesis in Asparagus racemosus. BMC Genomics 15, 746 (2014).