Transcriptome



Primary Details

Project ID PTP034
Transcriptome Exp. ID PTE0115
Plant Name Withania somnifera
Genome ID GEN2

Plant Classification

Kingdom Plantae
Phylum Streptophyta
Class Magnolipsida
Order Solanales
Family Solanaceae
Genus Withania
Species somnifera
Variety CWS11XCWS7

Transcriptome Details

Sample Tissue

whole plant

Line/Chemotype/Cultivar

CWS11XCWS7

Study Title

RNA seq analysis of CWS11x CWS74 F1hybrid of different Ecotype of Ashwagandha (Withania somnifera) genotypically adapted to specific environmental conditions

Description

To understand the genotypic adaptational polymorphisms in the two ecotypes of Ashwagandha i.e the dwarf, yellow berry, annual type and tall, red berry perennial, Kashmiri type, for their different response to their specific niche environment RNA seq expression profiling of one month old glass house grown plant of CWS7xCWS11F1 hybrid of different ecotype of Ashwagandha (Withania somnifera) genotypically adapted to specific environmental conditions was performed using Illumina HiSeq 2000 NEXTflex Directional RNA-Seq (dUTP based) strand specific libraries yielding 23 million processed reads assembling in 60477 contigs of an average N50 of 1687.

Sequencing Platform

Illumina HiSeq 4000

Sequencing Strategy

RNA-Seq

Sequencing Library

Paired

Experiment Type

Replicate Number

Total Replicates

Merged replicated

Library Name

CWS11XCWS7

Selection Method

PCR

Number of run

Spots No

27,003,085

Bases no

8.2G

Spot Length

302

Submitted By

NA

Other Related Entries

Project ID Experiment ID Plant Name Sample/Tissue Exp. Type Replicate No. Total Replicates No. Merged Assembly (Replicates) DEGs (Pair)
PTP034 PTE0114 Withania somnifera whole plant -
PTP034 PTE0115 Withania somnifera whole plant -
PTP034 PTE0116 Withania somnifera whole plant -