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| Publication text | Akhtar, M.Q., Qamar, N., Yadav, P., Kulkarni, P., Kumar, A. and Shasany, A.K. (2017), Comparative glandular trichome transcriptome-based gene characterization reveals reasons for differential (−)-menthol biosynthesis in Mentha species. Physiol Plantarum, 160: 128-141. https://doi.org/10.1111/ppl.12550 |
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| Abstract | The genes involved in menthol biosynthesis are reported earlier in Men-tha × piperita. But the information on these genes is not available in Men-tha arvensis. To bridge the gap in knowledge on differential biosynthesis ofmonoterpenes leading to compositional variation in the essential oil of thesespecies, a comparative transcriptome analysis of the glandular trichome (GT)was carried out. In addition to the mevalonic acid (MVA) and methylerythritolphosphate (MEP) pathway genes, about 210 and 196 different terpene syn-thases (TPSs) transcripts were identified from annotation in M. arvensis andM. × piperita, respectively, and correlated to several monoterpenes presentin the essential oil. Six isoforms of (−)-menthol dehydrogenases (MD), thelast enzyme of the menthol biosynthetic pathway, were identified, clonedand characterized from the transcriptome data (three from each species).Varied expression levels and differential enzyme kinetics of these isoformsindicated the nature and composition of the product, as these isoforms gen-erate both (−)-menthol and (+)-neomenthol from (−)-menthone and converts(−)-menthol to (−)-menthone in the reverse reaction, and hence together deter-mine the quantity of (−)-menthol in the essential oil in these two species.Several genes for high value minor monoterpenes could also be identifiedfrom the transcriptome data . |
| DOI | https://doi.org/10.1111/ppl.12550 |