FastQCFastQC Report
Wed 10 Jul 2024
D_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27418209
Sequences flagged as poor quality0
Sequence length50-159
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6330102.3087211859826438No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT846580.3087656090155269No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291180.10619949683803198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)